In the paper , both the size of the source and of the beam spot of a extended gaussian beam are considered and their influences on both the lateral and axial resolution of a fluorescent confocal microscopy are studied and calculated based on the power transfer function and three - dimensional pulse spread function 摘要研究一定光源孔徑、束斑的高斯光束對(duì)熒光共焦顯微鏡橫向、縱向分辨率的影響,導(dǎo)出了熒光功率傳輸函數(shù)、三維響應(yīng)函數(shù)。